Both the first and third terms are perfect squares.
$$ 25x^2 = \left( \color{blue}{ 5g } \right)^2 ~~ \text{and} ~~ 16 = \left( \color{red}{ 4 } \right)^2 $$The middle term ( $ -40x $ ) is two times the product of the terms that are squared.
$$ -40x = - 2 \cdot \color{blue}{5g} \cdot \color{red}{4} $$We can conclude that the polynomial $ 25g^{2}-40g+16 $ is a perfect square trinomial, so we will use the formula below.
$$ A^2 - 2AB + B^2 = (A - B)^2 $$In this example we have $ \color{blue}{ A = 5g } $ and $ \color{red}{ B = 4 } $ so,
$$ 25g^{2}-40g+16 = ( \color{blue}{ 5g } - \color{red}{ 4 } )^2 $$